1. What is Dynamic Light Scattering?

Dynamic Light Scattering (DLS) is a technique used to measure the size of particles in solution by analyzing the fluctuations in scattered light intensity caused by Brownian motion.

2. How Does DLS Work?

DLS measures the time-dependent fluctuations in scattered light intensity caused by particles moving under Brownian motion. The rate of these fluctuations is related to particle size.

Key parameters:

• Scattering intensity - Measured light intensity fluctuations
• Wavelength - Laser wavelength used in the measurement
• Temperature - Affects Brownian motion of particles
• Viscosity - Of the solvent used

3. Importance of Particle Size Measurement

Details: Particle size is crucial in many fields including pharmaceuticals, materials science, and biotechnology. It affects stability, bioavailability, and performance of products.

4. Using the Calculator

Tips: Enter all required parameters accurately. For best results, use values directly from your DLS instrument. Temperature should be in °C and viscosity in Pa·s.

5. Frequently Asked Questions (FAQ)

Q1: What size range can DLS measure?
A: Typically 0.3 nm to 10 μm, depending on instrument and sample properties.

Q2: How accurate is DLS?
A: Accuracy depends on sample preparation and instrument calibration. Typically ±2% for monodisperse samples.

Q3: What affects DLS measurements?
A: Temperature control, dust, air bubbles, and sample concentration can all affect results.

Q4: Can DLS measure polydisperse samples?
A: Yes, but interpretation is more complex and requires advanced analysis methods.

Q5: How does concentration affect DLS?
A: Too high concentration can cause multiple scattering; too low may give poor signal-to-noise ratio.