1. What is Cells Dilution?

Cell dilution is the process of reducing the concentration of cells in a solution by adding more solvent. This is commonly done in biological and medical laboratories to achieve the desired cell concentration for experiments or analyses.

2. How Does the Calculator Work?

The calculator uses the dilution formula:

Where:

• Initial Concentration — Original cell concentration (cells/mL)
• Dilution Factor — How many times the sample is diluted
• Final Concentration — Resulting cell concentration after dilution (cells/mL)

Explanation: The formula shows the inverse relationship between dilution factor and final concentration - the more you dilute, the lower the final concentration.

3. Importance of Cell Counting

Details: Accurate cell counting and dilution are essential for experiments requiring specific cell densities, such as cell culture, flow cytometry, and microbiology studies.

4. Using the Calculator

Tips: Enter the initial cell concentration in cells/mL and the dilution factor (must be ≥1). The calculator will determine the final concentration after dilution.

5. Frequently Asked Questions (FAQ)

Q1: What is a typical dilution factor used in cell culture?
A: Common dilution factors range from 2-fold to 100-fold, depending on the initial concentration and desired final concentration.

Q2: How do I prepare a 10-fold dilution?
A: Mix 1 part cell suspension with 9 parts diluent (e.g., 1 mL cells + 9 mL medium).

Q3: What's the difference between dilution factor and dilution ratio?
A: Dilution factor is the total volume divided by the sample volume (e.g., 1:10 dilution has a factor of 10). Dilution ratio expresses the parts (e.g., 1:9).

Q4: Should I account for cell viability in dilutions?
A: For most purposes, total cell count is used. However, viability should be considered for experiments sensitive to live/dead cell ratios.

Q5: How does serial dilution differ from simple dilution?
A: Serial dilution involves multiple successive dilution steps, each with its own dilution factor, resulting in an exponential decrease in concentration.