1. What is Cell Dilution Factor?

The Cell Dilution Factor (DF) is a dimensionless number that represents the ratio of the final volume to the initial volume when preparing cell suspensions or solutions. It indicates how much a sample has been diluted.

2. How Does the Calculator Work?

The calculator uses the dilution factor formula:

Where:

• \( Final\ Volume \) — Total volume after dilution (mL)
• \( Initial\ Volume \) — Original volume of undiluted sample (mL)

Explanation: The equation calculates the factor by which the original concentration has been reduced.

3. Importance of Dilution Factor

Details: Accurate dilution factor calculation is crucial for cell counting, preparing standard solutions, and ensuring proper concentrations in experiments.

4. Using the Calculator

Tips: Enter both volumes in mL. Both values must be positive numbers. The result is dimensionless.

5. Frequently Asked Questions (FAQ)

Q1: What does a DF of 10 mean?
A: A DF of 10 means the sample has been diluted 10-fold (1 part sample + 9 parts diluent).

Q2: How is DF different from dilution ratio?
A: DF is the inverse of dilution ratio. A DF of 10 corresponds to a 1:10 dilution ratio.

Q3: When should I use this calculation?
A: Use when preparing cell suspensions for counting, creating standard curves, or any serial dilution protocol.

Q4: What if my DF is less than 1?
A: A DF < 1 indicates concentration rather than dilution, which is unusual in standard protocols.

Q5: How precise should my volume measurements be?
A: Use the most precise measurement tools available (e.g., graduated cylinders or pipettes) for accurate results.