1. What is the Cell Dilution Formula?

The cell dilution formula calculates the final concentration of cells after dilution. It's fundamental in cell culture, microbiology, and laboratory procedures where precise cell concentrations are required.

2. How Does the Calculator Work?

The calculator uses the cell dilution formula:

Where:

• \( Final\ Concentration \) — Concentration after dilution (cells/mL)
• \( Initial\ Concentration \) — Starting concentration (cells/mL)
• \( Dilution\ Factor \) — Ratio of final volume to initial volume

Explanation: The formula shows that the final concentration is inversely proportional to the dilution factor.

3. Importance of Cell Dilution Calculation

Details: Accurate cell dilution is crucial for experiments requiring specific cell densities, such as plating cells, flow cytometry, or creating standard curves.

4. Using the Calculator

Tips: Enter initial cell concentration in cells/mL and the dilution factor (must be ≥1). The calculator will compute the final concentration.

5. Frequently Asked Questions (FAQ)

Q1: What's a typical dilution factor range?
A: Common dilution factors range from 2-1000x, depending on the application and initial cell density.

Q2: How do I determine the dilution factor?
A: DF = (Final Volume) / (Volume of Stock Solution). For example, adding 1mL cells to 9mL media gives DF=10.

Q3: Can I dilute multiple times?
A: Yes, serial dilutions are common. Multiply the individual dilution factors for total DF.

Q4: What if my cells are clumped?
A: Trypsinize or pipette vigorously to break clumps before counting and diluting for accurate results.

Q5: How precise should my measurements be?
A: Use calibrated pipettes and measure volumes carefully, especially for large dilution factors.